Soluble in alcohol, chloroform-soluble, water-soluble, non-soluble ether. Partially miscible with alcohol and glycerol. Crystal Violet Solution Version: 18 Solubility in water: Soluble. Dilute to a final volume of 100 ml with DI water. Crystal Violet (Ammonium Oxalate) According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 . One of the key events during the very early development of the new aniline dyes as biological stains was the introduction of the Gram stain (see Section 6.1.2). Compare this item. Due to differences in the thickness of a peptidoglycan layer in the cell membrane between Gram positive and Gram negative bacteria, Gram positive bacteria (with a thicker peptidoglycan layer . All products from TargetMol are for Research Use Only. Reaxys Registry Number. Concentration Solvent Mass 1 mg 5 mg 10 mg; 1 mM: 2.4511 mL: 12.2555 mL: 24.5110 mL: 5 mM: 0. . Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) ), indicator (pH 0.1-2.0) 46364 Crystal Violet, VETRANAL, analytical standard 32675 Crystal Violet, indicator for the determination of the redox potential, S. 344787. Water Solubility: 0.1 g/100 mL at 22.5 C MSDS. Draw the mechanism of formation of crystal violet starting with the formation of the Grignard reagent. Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin. Expired - Lifetime Application number US606284A Inventor Herrick Guy Scott No. The dye destroys cells and is used as a moderate-strength external disinfectant. Last Update2022-01-01 08:49:51. crystal violet o - Nature. At a pH of >1.0, the dye is green with absorption maxima at 420nm and . Product details 32.15 /Pack Qty. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines. Leucocrystal Violet has been reported that leucocrystal violet can be oxidized with acidified potassium iodate forming crystal violet dye, which shows a maximum absorbance at 590 nm. Source: Synthetic. Solvent wash all contaminated surfaces with 60-70% ethanol followed by washing with a soap and water solution. of water. Art. Product description: Crystal Violet is used as an acid-base indicator. Splash some acetone in. Description. Room temperature in continental US; may vary elsewhere. Add 0.02 g of crystal violet in 75 ml of DI water. If eye irritation persists: Get medical advice/attention. which are water-soluble and may spread in water systems. Crystal Violet According to Regulation (EC) No 1907/2006, Annex II, as amended. Target: Others Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. Solubility: Solubility in water: 16g/L (25C). MSDS # 237.00 Crystal Violet Solution, 0.1% Scholar Chemistry Section 9: Physical and Chemical Properties Molecular formula C 25 H 30 N 3 Cl. Reply:try chloroform or lots of hot water. The product contains volatile substances which may spread in the atmosphere. * In solvent : -80C, 6 months; -20C, 1 month (protect from light) Specific Gravity 1.00 g/mL @ 20C. Posted by Barbosa at 10:47 PM. Description. Gentian violet was first produced in the 19th century and was used for some time as an antibiotic and antifungal. Solubility Soluble in water. The emission wavelength is adjustable merely through the control of the solvent. Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. Contains: Crystal Violet 0.1% (CAS 548-62-9), .05% ethyl alcohol (CAS 64-17-5), 99.95% water (CAS 7732-18-15) Weber Scientific 2732 Kuser Road | Hamilton, NJ . It dissociates into positive and negative ions that can penetrate bacterial cells. Crystal violet (Basic Violet 3) is a triarylmethane dye. Gram staining. Solubility: 10 g/l: Dimensions; Materials Information; Toxicological Information; LD 50 oral: LD50 Rat 420 mg/kg: Safety Information according to GHS; Other solubilities: soluble in chloroform, 1 g/10 mL alcohol, 1 g/15 mL glycerin, practically insoluble in ether: Color: Green: CAS: 548-62-9,90-94-8: Dye Content: 88% min. It is mostly utilized as textile dye and paper dye and It can be used to treat itching in fish because of its antimicrobial properties. crystal violet,physical properties,suppliers,CAS,MSDS,structure,Molecular Formula, Molecular Weight ,Solubility,boiling point, melting point . Shop Thermo Scientific Crystal Violet, pure, indicator at Fishersci.co.uk English English; Change Country. Solubility: Alcohol and Water Synonyms: Gentian Violet, Basic Violet 3, C.I. Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei. Crystal Violet Preparation For Cell Staining: For crystal violet solution you dilute 0.125g of crystal violet in 50 ml of 20% methanol. Vapor Density (air=1) 0.7 (water). Gentian violet | C25H30ClN3 | CID 11057 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities . Specific Gravity/Density: 1 (Water=1) Molecular Formula: Not applicable. Solubility Testing; Filter Papers; Laboratory Seating; Laboratory Supplies, General. Riboflavin is photosensitive to UV and visible light in terms of . Targets Solubility * Preparing Stock Solutions * The above data is based on the productmolecular weight 407.98. from 24 Pack Qty. Crystal Violet is used as an acid-base indicator, alcohol denaturant, biological stain, textile dye, and an indicator for copper salts. Crystal Violet Biological Activity. Soluble in water (50 mg/ml at 27 C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Leucocrystal violet is a triphenylmethane dye which can be used to detect antimony in environmental and biological samples using spectrophotometric techniques. Crystal Violet, 1% w/v in Glacial Acetic Acid Safety Data Sheet according to Federal Register / Vol. The structural formula of its chloride is: crystal violet dye solvent solubility double salt Prior art date 1945-07-20 Legal status (The legal status is an assumption and is not a legal conclusion. The protonated form (found in acidic conditions) is yellow, turning blue-violet above . Solubility: N/A Decomposition Temperature: N/A 10. Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol. -Crystal Violet is a non-toxic alternative, used in place of the fluorescent dye ethidium bromide in DNA gel electrophoretic runs. Crystal violet is a blue monochloride salt of crystal violet cation. Waste water containing CV may affect aquatic ecosystems. Cation-based violet dye. Its solubility in water is 1.68% and its solubility in 95% ethanol is 13.87%. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with . Reagent and Dye Solubility Chart Reagent and Dye Solubility Chart Roy C. Ellis IMVS Division of Pathology The Queen Elizabeth Hospital Woodville Road, Woodville, South Australia 5011 Email: roy.ellis@imvs.sa.gov.au This is to be used as a guide only as solubility data varies between manufacturers for the same product especially for dyes)1-3 The concentration of the prepared solution is about 05.00 gram/liter. This problem has been solved! . in 30 to 40 cc. Physical State (20 deg.C) Solid. Crystal Violet, CAS 548-62-9 Bulk Inquiry Add to Cart Select Batch Purity 100.00% LCMS COA Datasheet SDS Cite this Article. Related Products: Crystal Violet Stain. . Prepare Crystal Violet Stain. First Aid Measures Eye Contact: If in eyes: Rinse cautiously with water for several minutes. Other solubilities: soluble in chloroform, 1 g/10 mL alcohol, 1 g/15 mL glycerin, practically . gives the solubility of the dye as 1 Gm. It has been used in creams for the topical treatment of bacterial and fungal infections, being effective against some Gram-positive bacteria (notably Staphylococcus species) and some pathogenic fungi (including Candida species) but use declined following reports of animal carcinogenicity. Insoluble in ether. Crystal Violet, 1% 1. Thermo Scientific Crystal Violet, certified . No. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Basically you need to workout what it's soluable in and use that solvent. The colour of the dye depends on the acidity of the solution. Product Application: Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. 1552-42-7. Chemistry questions and answers. 42555. Solubility Miscible with water and chloroform. Use absorbent paper dampened with 60-70% ethanol to pick up any remaining material. Crystal violet (C.I. CRYSTAL VIOLET TS C900.7E INTEGRA Chemical Company24 Hour Emergency Response: CHEMTREC 800-424-9300 (Outside USA 703-527-3887) Conclusions. Melting . Crystal violet lactone MSDS(Chinese) Safety Information. Synonyms: Hexamethylpararosaniline Chloride; Gentian Violet; Basic Violet 3; Methyl Violet 10B; Pyoctanin; C.I. When the purple crystal is dissolved in water, it will turn blue or purple. Crystal violet is a triarylmethane dye. Crystal Violet Dye content 90 %; CAS Number: 548-62-9; EC Number: 208-953-6; Synonyms: Basic Violet 3,Gentian Violet,Hexamethylpararosaniline chloride,Methyl Violet 10B; Linear Formula: C25H30N3Cl; find Sigma-Aldrich-C3886 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich CAS RN. 42555) CAS 548-62-9 indicator ACS,Reag. 77, No. 4. In addition to the stain itself, the solution generally contains phenol to aid the absorption of the stain by the sebaceous material in the fingermark. However, the solubility of its iodide in two solvents is only 0.035% and 1.78% respectively. SECTION 1: Identification of the substance/mixture and of the company/undertaking . For lysing solution, dilute 5.88 g of sodium . Molecular weight 407.99. Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. The product contains volatile substances which may spread in the atmosphere. Newer Post Older Post Home. Crystal violet (CV) is applied in daily use mainly as a commercial dye and antimicrobial agent. Concentration: 0.2% w/v in Glacial Acetic Acid Composition: Acetic Acid 99.81%, Gentian Violet 0.19% Boiling Point: Approximately 118C Density: 1.05 Melting Point: 16.64C Color: Dark blue to violet/purple liquid Physical State: Liquid Flash Point: 39C pH Range: Solubility. Remove contact lenses, if present and easy to do. Crystal Violet. Its photodynamic action is elicited via a free-radical mechanism. This aniline-derived dye has various antimitotic and antifungal properties. No comments: Post a Comment. Crystal violet (CV) dye is triphenylmethane cationic dye (figure 1). Riboflavin, also known as vitamin B 2, is non-toxic and an essential vitamin required for the functions of the human body. * "" means soluble, but saturation unknown. Continue rinsing. Sparingly Soluble in Water, Freely Soluble in Ethanol (96%) and In Methylene Chloride: Passes Test: 3: Identification . Question: Why are serial dilutions required, and not just a simple dilution for visible spectrophotometry? Crystal violet Cell Culture Tested Product Code: TC510 Disclaimer : User must ensure suitability of the product(s) in their application prior to use. Symbol: GHS07: Signal Word: Warning . When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M-1 cm-1. However, physicians prescribe solutions of the dye in con The compound helps to assess bacterial contamination of tissue culture samples. Crystal violet is insoluble in ether, it is soluble in water, chloroform and alcohol. Therefore, this method can realize sensitive detection of crystal violet. Concentration: 0.2% w/v in Glacial Acetic Acid Composition: Acetic Acid 99.81%, Gentian Violet 0.19% Boiling Point: Approximately 118C Density: 1.05 Melting Point: 16.64C Color: Dark blue to violet/purple liquid Physical State: Liquid Flash Point: 39C pH Range: Solubility. Molecular Weight: No information found Section 10 - Stability and Reactivity Chemical Stability: Stable under normal temperatures and pressures. Soluble in water to 25 mM, in DMSO to 100 mM and in ethanol to 10 mM. Solubility ethanol Absorption maximum Colour Empirical formula Formula weight: Crystal violet Crystal violet Gentian violet Methyl violet 10B 42555 Basic violet 3 Triarylmethane Basic 1.68% 13.87% 593 (Gurr), 588 (Aldrich) Blue violet C 25 H 30 N 3 Cl 408: Crystal violet is the deepest blue of all the methyl violets, and is satisfactory for . Crystal Violet (Gentian Violet) has antiviral effects against H1N1 and also has prominent bactericidal activities. Crystal violet (C.I. BuyersGuideChem provides reliable information about Crystal violet (548-62-9) like chemical properties, structure, melting point density, molecular formula, molecular weight, physical properties, toxicity information. The compound helps to access bacterial contamination of tissue culture samples. Notes Leucocrystal Violet | C25H31N3 | CID 69048 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities . 6.3. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water. It is used in textile and paper dye industries as well as navy blue and black inks for printing, ball-point pens and inkjet printers. Crystal Violet has antibacterial and antifungal properties. Gentian violet, also known as crystal violet and methyl violet 10B, is a blue-violet dye. A staining solution containing gentian violet ( crystal violet) may be employed for the detection of the fatty components in the latent fingerprint deposit. Question: How does the low pH affect the solubility of the crystal violet? . From these solubility figures it is evident that it would be impossible to make an aqueous solution of gentian violet in a concen tration of more than about 3 per cent. 42555 Shelf Life: 36 Months. An intense purple coloration results. Used as a component of CV cytotoxicity assays. Conditions to Avoid: Incompatible materials, ignition sources . The compound helps to access bacterial contamination of tissue culture samples. Water Solubility: 16 g/L (25) Physical and Chemical Properties: melting point 215C water-soluble 16g/L (25C) Use: Used as a biological stain: . The colour of the dye depends on the acidity of the solution. Generally, it requires a strong acid such as sulfuric acid eliminate a hydroxy group. Identification . Science; Chemistry; Chemistry questions and answers; How does the low pH affect the solubility of the crystal violet? T123.1 In stock Delivery fast, simple and reliable! Why is crystal violet soluble? Subscribe to: Post Comments (Atom) Chemical Name CAS Number 1DPH 54 CERCLA RQ 734 CAA 112(2) TQ Crystal Violet 548-62-9 No No No No No Section 16 Additional Information Revised: 09/03/2014 Replaces: 09/03/2014 Printed: 04-21-2015 This paper investigated the preparation of CDs with 1,2,4,5-tetraaminobenzene as the raw material. It is known in medicine as Gentian violet (or crystal violet or pyoctanin (e) [2]) and is the active ingredient in a Gram stain, used to classify bacteria. Once it is dissolved, dilute to 100.00 ml with acetic acid. Products conform solely to the information contained in this and other . Not for Human or Veterinary or Therapeutic Use. 58 / Monday, March 26, 2012 / Rules and Regulations Date of issue: 12/30/2014 Revision date: 05/03/2017 Supersedes: 09/14/2015 Version: 1.2 05/03/2017 EN (English US) Page 1 SECTION 1: Identification 1.1. Soluble in water 50 g / L at 27C. Crystal violet or methyl violet is used in many applications, including: As a pH indicator (yellow to violet with the transition at a pH = 1.6) In the medical community, it is the active ingredient in Gram's Stain, used to classify bacteria. It is a component of gram staining in microorganisms and cell lines. Appearance Dark blue-violet liquid. Crystal Violet assay protocol summary: - remove culture medium - wash cells - add Crystal Violet staining solution . A component used in gram staining as a counter-stain to Safranin O (Prod # S7400). from 6 Pack Qty. For use as a stain for bacteria, Add 2 g of crystal violet to 20 ml of 95% ethyl alcohol. Articles of Crystal violet lactone are included as well. but is now mostly regarded as a synonym for crystal violet. Crystal/gentian violet. Leucocrystal Violet is a triphenylmethane dye used in the detection of antimony in environmental and biological samples using spectrophotometric techniques. How can the solubility of crystal violet be increase to clean the glassware? It is also used to colourize diverse products such as fertilizers, antifreeze, detergents and leather. . Odor Threshold N/A. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590 nm and an extinction coefficient of 87,000 M 1 cm 1 . 2. excl. Both its aqueous solution and alcohol solution are purple. 30.54/Pack Qty. Insoluble . It is soluble in water, alcohol and chloroform, but insoluble in ether. How do I clean crystal violet from a beaker? Crystal Violet, certified by the Biological Stain Commission C6158 Crystal Violet, ACS reagent, 90.0% anhydrous basis 61135 Crystal Violet, for microscopy (Bact., Bot., Hist., Vit. : 785 Odor No odor. Why would water be a strong enough acid to eliminate the hydroxy in this . No. If using pH 7.0 water fails to improve solubility, I would suggest dissolving the Crystal violet first in 0.25% methanol, and then diluting this in water to a working concentration of 0.05%. Get All The Latest News Help & Support Center English English; Change Country; 0 . Besides, crystal violet also shows good solubility in ethanol. Ph Eur - Find MSDS or SDS, a COA, data sheets and more information. Thermo Scientific Crystal violet, ACS reagent Manufacturer: Thermo Scientific 447570500 View more versions of this product Catalog No. The use of gentian violet (crystal violet) as a bacterial stain led, eventually, to its use as an antibacterial. Crystal Violet 548-62-9 Trade Secret Water 7732-18-5 Trade Secret Ammonium Oxalate 6009-70-7 Trade Secret 4. Crystal Violet Page 4 of 4 Section 15 Regulatory Information TSCA Status: All components in this product are on the TSCA Inventory. Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. . Form / State: Solid. Stability and Reactivity Reactivity: Chemical Stability: Stable Conditions of Stability/Instability: N/A . 42555) for microscopy Certistain, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. Methods and material for containment and cleaning up The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead". . Solubility 20 ppm in water yields clear dark purple solution Absorption maxima 580.00 -595.00 Loss on drying (at 110C . water-soluble and may spread in water systems.
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